The analysis of proteinaceous artists' media by high performance liquid chromatography - Tate Research Repository
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Journal article

The analysis of proteinaceous artists' media by high performance liquid chromatography

1997

Abstract

This paper details the development and use of a reverse phase high performance liquid chromatographic (RP-HPLC) technique for the am/no acid analysis of proteinaceous artists' media. Samples (for example, animal glues, milk and egg proteins) were hydrolysed under acid conditions, then derivatized with 9-fluorenyl-methyl chloroformate (FMOC). Analysis was achieved via injection onto a Spherisorb ODS-2 (Phase Separations Ltd, Deeside, Flintshire, UK) column with a gradient elution programme and ultraviolet (UV) detection. Samples of proteinaceous binding materials removed from works of art have been successfully identified using the method described.

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Metadata

  • Resource type

    Journal article

  • Institution
    • Tate

  • Journal title
    • LC GC Europe

  • Volume
    • 10

  • Issue
    • 1

  • Pagination
    • 48-53

  • Publisher
    • Advanstar Communications Inc.

  • ISSN
    • 1471-6577