The analysis of proteinaceous artists' media by high performance liquid chromatography

Vallance, S.L.; Singer, B.W.; Hitchen, S.M.; Townsend, Joyce H.

The analysis of proteinaceous artists' media by high performance liquid chromatography

Resource type

Journal article

Creator (person)

Vallance, S.L.

Singer, B.W.

Hitchen, S.M.

Townsend, Joyce H.

Date published

1997

Abstract

This paper details the development and use of a reverse phase high performance liquid chromatographic (RP-HPLC) technique for the am/no acid analysis of proteinaceous artists' media. Samples (for example, animal glues, milk and egg proteins) were hydrolysed under acid conditions, then derivatized with 9-fluorenyl-methyl chloroformate (FMOC). Analysis was achieved via injection onto a Spherisorb ODS-2 (Phase Separations Ltd, Deeside, Flintshire, UK) column with a gradient elution programme and ultraviolet (UV) detection. Samples of proteinaceous binding materials removed from works of art have been successfully identified using the method described.

Journal title

LC GC Europe

Volume

10

Issue

1

Publisher

Advanstar Communications Inc.

ISSN

1471-6577